Long read sequencer covers gaps in understanding
The Pacific Biosystems Sequel System is based on the same SMRT cell technology as its predecessor, the RS II. The Sequel features one million zero mode waveguides per SMRT cell vs 140,000 in the RS II. This 7-fold increase speeds up projects and reduces the cost per genome.
PacBio has also decreased the footprint and weight of the system to less than one third of the RS II. It’s now more attractive for laboratories where space is limited.
Advantages of Long Read Sequencing
Long read sequencing (avg. read length 10 Kb) solves many of the challenges inherent in short read sequencing. You can take advantage of this to cover the gaps in short read assemblies, understand variant phasing (which alleles are on which chromosome) and detect base modifications in one reaction.
Modified bases e.g. 5mC, are identified by changes in the kinetics of incorporation at the same time as the genomic sequence is gathered.
You can even sequence full length mRNAs (by cDNA) for transcripts up to 10Kb to detect transcript isoforms and alternate start sites. Or measure allele specific gene expression without assembly.
Long reads also help ensure you don’t miss large scale rearrangements or low complexity regions.
- Whole genome sequencing
- Genome assembly with variant phasing
- Transcriptome sequencing
- Targeted sequencing
Sequel System Features
- Cover genomic regions inaccessible to other sequencing technologies.
- 10Kb Average read length
- >99.999% Consensus accuracy
- Detect base modifications and sequence in one reaction
- Reveal variant phasing
- Low GC context bias
- U.S. List Price: ~$350,000
Sequel System Specifications
- Library prep in 6 hours
- Run Time: User selected – 0.5-6 hours/SMRT cell
- Run Size: User selected – 1-16 SMRT cells allows flexibility to manage reagent consumption
- 8X -50X scalable sequence coverage dependent on application